James R. Mansfield
Summary of Qualifications
· 20 years of experience in biomedical imaging and spectroscopy application and instrumentation development, familiar with the instrumentation and applications of all pre-clinical in vivo imaging modalities and the vast majority of microscopy imaging modalities.
· 45 publications and 7 issued patents to date; associate editor of the American Journal of Nuclear Medicine and Molecular Imaging; invited speaker and session chair for a variety of small animal imaging conference sessions.
· Product manager and senior applications scientist for the Nuance™ and Maestro™ imaging systems, each with over 20% CAGR in sales from 2004 to present.
· Extensive experience in imaging experiments, including the choice of appropriate imaging methods, implementation of a range of image analysis methods and with experimental design which will enable the extraction of statistically relevant data from the images.
· Involved with all aspects of the hardware and software design, development, sales, customer training and application development for the CRi Maestro™ in vivo imaging system, winner of R&D Magazine’s Top 100 Inventions of 2005 award.
· Primary developer and thought-leader for commercial in vivo pharmacokinetic fluorescence imaging methodologies (DyCE™, or Dynamic Contrast Enhancement) for IVIS™ and Maestro™ imaging systems
PERKINELMER (PKI) / CALIPER LIFE SCIENCES (CALP) 2010-present
Director, Tissue Analysis Applications
· Senior applications scientist for the Nuance, TRIO, inForm and Vectra microscopy imaging product lines
· Responsible for system and software applications and sales training for direct North American sales staff and world-wide distributor network for the microscopy and Maestro small animal imaging product lines
· Senior speaker for public speaking engagements at conferences, workshops, regional symposiums and local colloquia, and for technical sales and applications presentations to prospective customers around the world.
· Working with customers to develop key application areas for our instrumentation, including the multiplexed imaging and quantitation of miRNA and its putative target protein and a fluorescence imaging transplantation graft assessment methodology
CAMBRIDGE RESEARCH & INSTRUMENTATIon (CRi) 2004-2010
Director, Multispectral Imaging Systems
· Product manager and senior applications scientist for the Nuance™ and Maestro™ imaging systems, each with over 20% CAGR in sales from 2004 to present. Responsible over the years for virtually all aspects of the instruments, including hardware, software and application development, after-sales support, customer and sales staff training and marketing materials.
· Recipient of numerous awards, including: The Scientist Magazine Top 10 Innovations for 2010 (Maestro Dynamic); Frost & Sullivan US Optical Imaging Technologies in Drug Discovery Product Line Strategy Award, 2009; SPIE and Laurin Publishing Prism Award Finalist, 2009 (Maestro with DyCE); Cleveland Clinic Top 10 Medical Innovations, 2008 (Nuance); R&D Magazine’s R&D 100 Award, 2005 (Maestro).
· Participated in the writing, work and reporting for numerous NIH grants for both in vivo imaging and microscopy, including one to develop an optical tomographic 3D fluorescence imaging system (“Maestro 3D”) and one to commercialize the in vivo pharmacokinetic fluorescence imaging methodologies (“DyCE”)
HYPERMED, INC. 2002-2004
Chief Technology Officer
· Co-founder and sole full-time employee of a small start-up company commercializing skin oxygenation imaging systems for burn and wound therapy, diabetic foot ulcer management and non-invasive shock assessment
· Responsible for all aspects of the technology for the company from conceptualization to implementation of imaging systems for pre-clinical trials.
· Principal investigator for three funded Department of Defense grant proposals for the use of hyperspectral imaging in shock monitoring
ARGOSE, INC. 1999-2002
Director of Analysis
· Key scientist in a large team of scientists researching the methodologies necessary for the development of a practical non-invasive glucose monitor for home consumer use.
· Responsible for the management, development, hiring, and growth as well as the research goals of the Analysis Group, which grew to be a nine-person group, including five direct reports.
· A member of the senior management team at Argose, responsible for developing and working to meet timelines, research plans, budgets, and also to provide the majority of the scientific data that was required in support of fund raising efforts..
· Worked closely with the engineering department at Argose during the development of research-grade spectrometers and prototype instrumentation, providing key input on analytical methodologies, spectroscopic trade-offs and data handling issues necessary to optimize the ability to measure glucose in vivo.
NATIONAL RESEARCH COUNCIL OF CANADA, INSTITUTE FOR BIODIAGNOSTICS 1993-1999
Technical Officer, TO-4
· Designed, developed and implemented practical multispectral imaging systems for human and animal studies using a novel liquid crystal tunable filter (LCTF) technology.
· Assisted and collaborated with IBD researchers who needed multivariate analysis methodologies to analyze their spectroscopic and imaging data.
· Co-developed a research program for the use of multispectral imaging in burn and wound assessment and the imaging of works of art.
HEALTH AND WELFARE CANADA 1991-1993
· Worked as an analytical chemist performing identification analyses of drugs of abuse for courtroom testimony. This included all of the analysis, calibration, SOPs and other documentation necessary for a validated presentation before the court.
· Very familiar with many analytical instrumentation techniques, including HPLC, GC, GC-MS, FT-IR and NMR.
MSc, Chemistry, University of Manitoba, Winnipeg, 1991
BSc, Chemistry and Biology, University of Winnipeg, Winnipeg, 1989
46) Lei Zhu, Ning Guo, Quanzheng Li, Ying Ma, Orit Jacboson, Seulki Lee, Hak Soo Choi, James R Mansfield, Gang Niu, Xiaoyuan Chen “Dynamic PET and Optical Imaging and Compartment Modeling using a Dual-labeled Cyclic RGD Peptide Probe.” Theranostics. 01/2012; 2(8):746-56.
45) Elizabeth M. C. Hillman, Cyrus B. Amoozegar, Tracy Wang, Addason F. H. Mccaslin, Matthew B. Bouchard, James Mansfield, Richard M. Levenson “In vivo optical imaging and dynamic methods for biomedical research” Philosophical transactions. Series A, Mathematical, physical, and engineering sciences. 11/2011; 369(1955):4620-43.
44) Mansfield, J.R. “Cellular context in epigenetics: Quantitative multicolor imaging and automated per-cell analysis of miRNAs and their putative targets” Methods 52 (2010) 271–280.
43) Mansfield, J. “Distinguished Photons: A Review of In Vivo Spectral Fluorescence Imaging in Small Animals” Current Pharmaceutical Biotechnology 2010, 11,628-638.
42) James R. Mansfield and Richard M. Levenson “Paving a New Path: Multispectral Imaging in Pathology” inFocus Magazine, June 2009.
41) James R. Mansfield, Clifford Hoyt, and Richard M. Levenson “Visualization of Microscopy-Based Spectral Imaging Data from Multi-Label Tissue Sections” Current Protocols in Molecular Biology 14.19.1-14.19.15, October 2008.
40) Bouchard MB, MacLaurin SA, Dwyer PJ, Mansfield J, Levenson R, Krucker T. “Technical considerations in longitudinal multispectral small animal molecular imaging.” J Biomed Opt. 2007 Sep-Oct;12(5):051601.
39) Levenson, Richard M.; Mansfield, James R. “Multispectral imaging in medicine and biology: Slices of life”, Cytometry A. 2006 Aug 1;69(8):748-58.
38) Leopoldo C. Cancio, MD; Andriy I Batchinsky, MD; James R Mansfield, MSc; Svetlana Panasyuk, PhD; Katherine Hetz, BS; David Martini, MS; Bryan S Jordan, RN, MSN; Brian Tracey, PhD; Jenny E Freeman, MD “Hyperspectral Imaging: A New Approach to the Diagnosis of Hemorrhagic Shock”, J. Trauma, 2006 May;60(5):1087-95.
37) James R. Mansfield, Clifford C. Hoyt, Peter J. Miller, Richard M. Levenson “Distinguished photons: increased contrast with multispectral in-vivo fluorescence imaging”, Biotechniques, Vol. 39 Supplement: pp S25-S29 (Dec 2005).
36) Mansfield, James R.; Gossage, Kirk W; Hoyt, Clifford; Levenson, Richard “Autofluorescence removal, multiplexing and automated analysis methods for in-vivo fluorescence imaging” Journal of Biomedical Optics, 2005 Jul-Aug; 10(4): 41207.
35) Gillies, Robert; Levin, Robin K.; Mansfield, James R.; Hopmeier, Michael J.; Freeman, Jenny E. “Use of hyperspectral imaging to profile cutaneous responses to bioweapons: a concept paper” Proc SPIE, Vol 5072, pp. 32-44 (2003).
24) Kellicut Dwight C.; Weiswasser Jonathan M.; Arora Subodh; Freeman Jenny E.; Lew Robert A.; Shuman Charles; Mansfield James R.; Sidawy Anton N. “Emerging technology: Hyperspectral imaging” Perspectives in vascular surgery and endovascular therapy, 2004, vol. 16, no. 1, pp. 53-57.
33) J.M.G. Canvin, S. Bernatsky, C.A. Hitchon, M. Jackson, M.G. Sowa, J.R. Mansfield, H.H. Eysel, H.H. Mantsch, H.S. El-Gabalawy, “Infrared spectroscopy: shedding light on synovitis in patients with rheumatoid arthritis” Rheumatology (Oxford), 2003 Jan 42:76-82.
32) Michael Attas, Edward Cloutis, Douglas Goltz, Claudine Majzels, James R. Mansfield, Henry H. Mantsch “Spectroscopic Imaging in Art Conservation: A New Tool for Materials Investigation” Leonardo: Journal of the International Society for the Arts, Sciences and Technology Volume 36, Number 4, 2003, 304-307.
31) Robert Gillies, Jenny E. Freeman, Leopoldo C. Cancio, Derek Brand, Michael Hopmeier, James R. Mansfield “Systemic Effects of Shock and Resuscitation Monitored by Visible Hyperspectral Imaging” Diabetes Technology & Therapeutics, Volume 5, Number 5, 2003, 847-855.
30) Attas, M.; Cloutis, E.; Collins, C.; Goltz, D.; Majzels, C.; Mansfield, J.R.; Mantsch, H.H., “Near-infrared spectroscopic imaging in art conservation: investigation of drawing constituents” Journal of Cultural Heritage, Volume 4, Issue 2, April 2003, Pages 127-136.
29) S.P. Nighswander-Rempel, R.A. Shaw, J.R. Mansfield, M. Hewko, V.V. Kupriyanov, H.H. Mantsch, "Regional Variations in Myocardial Tissue Oxygenation Mapped by Near-Infrared Spectroscopic Imaging," Journal of Molecular and Cellular Cardiology, 34, 1195-1203 (2002).
28) Leopoldo C. Cancio, Derek Brand, Jeffrey Kerby, Jenny E. Freeman, Michael Hopmeier, James R. Mansfield “Visible Hyperspectral Imaging: Monitoring The Systemic Effects of Shock and Resuscitation” Proc. SPIE, Vol. 4614, 159-168.
27) L.M. McIntosh, M. Jackson, H.H. Mantsch, J.R. Mansfield, A.N. Crowson, and J.W.P. Toole. "Near-Infrared Spectroscopy For Dermatological Applications," Vibrational Spectroscopy, 28, 53-58, 2002.
26) J.R. Mansfield, M. Attas, C. Majzels, E. Cloutis, C. Collins, and H.H. Mantsch, "Near Infrared Spectroscopic Reflectance Imaging: A New Tool in Art Conservation," Vibrational Spectroscopy, 28, 59-66, 2002.
25) L.M. McIntosh, J.R. Mansfield, A.N. Crowson, M. Jackson, H.H. Mantsch, “Infrared Imaging of Skin Lesions” Proc. SPIE, Vol. 4577, 226-229, 2002.
24) Laura M. McIntosh, Randy Summers, Michael Jackson, Henry H. Mantsch, James R. Mansfield, Marilyn Howlett, A. Neil Crowson, and John W. P. Toole “Towards Non-Invasive Screening of Skin Lesions by Near-Infrared Spectroscopy” Journal of Investigative Dermatology 116:175-181, 2001.
23) R.A. Shaw, J.R. Mansfield, S.P. Rempel, S. Low-Ying, V.V. Kupriyanov, & H.H. Mantsch. “Analysis of Biomedical Spectra and Images: From Data to Diagnosis.” Journal of Molecular Structure (Theochem), 500, 129-138 2000.
22) R. Salzer, G. Steiner, H.H. Mantsch, J. Mansfield, & N.E. Lewis. “Infrared and Raman Imaging of Biological and Biomimetic Samples.” Fresenius Journal of Analytical Chemistry, 366, 712-726 2000.
21) R.A. Shaw, J.R. Mansfield, V.V. Kupriyanov, & H.H. Mantsch. “In vivo Optical/Near-Infrared Spectroscopy and Imaging of Metalloproteins.” Journal of Inorganic Biochemistry, 79, 285-293 2000.
20) J.R. Mansfield, M.G. Sowa, & H.H. Mantsch. Development of LCTF-based Visible and Near-IR Spectroscopic Imaging Systems for Macroscopic Samples. Proc SPIE, 3920, 99-107 2000.
19) L.M. McIntosh, J.R. Mansfield, A.N. Crowson, J.W.P. Toole, H.H. Mantsch, & M. Jackson. Classification of Infrared Spectra from Skin Tumours. Proc SPIE, 3907, 126-135 2000.
18) Mansfield, J.R.; McIntosh, L.M.; Jackson, M.; Crowson, A.N.; Mantsch, H.H. "Search Engines for Infrared Microscopic Maps: Linear Discriminant Analysis using an External Training Set" Applied Spectroscopy, 53, 1323-1330 1999.
17) McIntosh, L.M.; Mansfield, J.R.; Crowson, N.; Mantsch, H.H.; Jackson, M. “Analysis and Interpretation of Infrared Microscopic Maps: Visualization and Classification of Skin Components” Biospectroscopy, 265-275 1999.
16) Jackson, M.; Mansfield, J.R.; Dolenko, B.; Somorjai, R.L.; Mantsch, H.H.; Watson, P.H. “Prediction of Breast Tumor Grade and Steroid Receptor Status by Multivariate Analysis of Fourier Transform Infrared Spectra” Cancer Prevention and Detection, 23, 245-253, 1999.
15) Mansfield, J.R.; Mazjels, C; Collins, C.; Cloutis, E.; Mantsch, H.H. “Near Infrared Spectroscopic Reflectance Imaging: Supervised versus Unsupervised Analysis Using an Art Conservation Application” Vibrational Spectroscopy, 19, 33-45, 1999.
14) Mansfield, J.R.; Sowa, M.G.; Mantsch, H.H. “Near Infrared Spectroscopic Reflectance Imaging: Methods for Functional Imaging and In-Vivo Monitoring” Proc SPIE. 3597, 222-233 1999.
13) Mansfield, J.R.; Sowa, M.G.; Payette, J.; Badur; Stranc, F.; Mantsch, H.H. “A Fuzzy C-Means Clustering Approach to the Analysis of Multispectral Images of the Rat Dorsal Skin Flap” IEEE Transactions in Medical Imaging, 6: 1011-1018, 1998.
12) M.G. Sowa, J.R. Payette, M.D. Hewko, J.R. Mansfield, H.H. Mantsch “Hemodynamic information obtained by statistical analysis of near-IR spectroscopic images” Proc SPIE. (Infrared Spectroscopy: New Tool in Medicine) 3257: 296-306, 1998.
11) M. Jackson, K. Kim, J. Tetteh, J.R. Mansfield, B. Dolenko, R.L. Somorjai, F.W. Orr, P.H. Watson, H.H. Mantsch “Cancer diagnosis by infrared spectroscopy: methodological aspects” Proc SPIE (Infrared Spectroscopy: New Tool in Medicine) 3257: 24-34, 1998.
10) Mansfield, J.R.; Sowa, M.G.; Scarth, G.B.; Mantsch, H.H. “The Use of Fuzzy C-Means Clustering in the Analysis of Spectroscopic Imaging Data” Analytical Chemistry, 1997, 69, 3370-3374.
9) Mansfield, J.R.; Sowa, M.G.; Scarth, G.B.; Somorjai, R.L.; Mantsch, H.H. “Fuzzy C-Means Clustering and Principal Component Analysis of Time Series from Near-Infrared Imaging of Forearm Ischemia” Computerized Medical Imaging and Graphics, 21:5, 1997, 299-308.
8) M.G. Sowa, J.R. Mansfield, M. Jackson, J.C. Docherty, R. Deslauriers, and H.H. Mantsch, "FT-IR/NIR Assessment of Ischemic Damage in the Rat Heart" Mikrochimica Acta [suppl.], 1997, 14, 451-453.
7) M.G. Sowa, J.R. Mansfield, G.B. Scarth, H.H. Mantsch "Noninvasive Assessment of Regional and Temporal Variations in Tissue Oxygenation by Near Infrared Spectroscopy and Imaging" Applied Spectroscopy 1997, 51:2, 143-152.
6) Mantsch, H.H., Eysel, H., Jackson, M., Liu, K.-Z., Mansfield, J.R., Schultz, C., Shaw, R.A. and Sowa, M. “Emerging medical applications of infrared technologies.” In Spectroscopy of Biological Molecules: Modern Trends” (Carmona, P., Navarro, R. and Hernanz, R.A., Eds.): Kluwer Academic Publ., 1997, 421-424
5) Loeb, Stephen J; Mansfield, James R “Platinum(II) complexes of the tridentate thioether ligands RS(CH2)3S(CH2)3SR (R = Et, i-Pr, Ph). Structures of [PtCl(i-PrS(CH2)3S(CH2)3 Si-Pr)][BF4], [PtI(PhS(CH2)3S(CH2)3SPh)][BF4], and [Pt(PPh3)(i-PrS(CH2)3S(CH2)3Si-Pr)][BF4]2•CH2Cl2” Canadian Journal of Chemistry, 1996, 74:7, 1377-91.
4) L.-P. Choo, J.R. Mansfield, N. Pizzi, R.L. Somorjai, M. Jackson, W.C. Halliday, and H.H. Mantsch, “Infrared Spectra of Human Central Nervous System Tissue: Diagnosis of Alzheimer's Disease by Multivariate Analysis” Biospectroscopy 1995, 1, 141-148.
3) N. Pizzi, L.-P. Choo, J.R. Mansfield, M. Jackson, W.C. Halliday, H.H. Mantsch, and R.L. Somorjai “Neural Network Classification of Infrared Spectra of Control and Alzheimer's Disease Tissue” Artificial Intelligence in Medicine 1995, 7, 67-79.
2) L.-P. Choo, J.R. Mansfield, N. Pizzi, R.L. Somorjai, M. Jackson, W.C. Halliday, and H.H. Mantsch “Classification of Infrared Spectra of Alzheimer's Disease by Multivariate Analysis” Spectroscopy of Biological Materials, J.C. Merlin et al. (eds.), Kluwer Academic Publishers 1995, 587-488.
1) Schaefer, T., Mansfield, J.R., Hruska, F.E., Sebastian, R., “The solvent dependence of the small internal barrier to rotation in 3,5-dichlorobenzyl fluoride.” Canadian Journal of Chemistry 1992, 70, 1750-1753.
1. US #7,899,518 Non-invasive tissue glucose level monitoring
2. US #7,289,835 Multivariate analysis of green to ultraviolet spectra of cell and tissue samples
3. US #7,013,172 Hyperspectral imaging calibration device
4. US #6,810,279 Hyperspectral imaging calibration device
5. US #6,721,582 Non-invasive tissue glucose level monitoring
6. US #6,640,130 Integrated imaging apparatus
7. US #6,424,859 Diagnosis of rheumatoid arthritis in vivo using a novel spectroscopic approach
Published Patent Applications
1. US 20040236229 Integrated imaging device
2. EP 1226462 Interface medium for tissue surface probe
3. WO 03010510 Adjunct quantitative system and method for non-invasive measurement of in vivo analytes
4. WO 03010561 Optical coupler for measuring tissue fluorescence
5. US 20010034477 Multivariate analysis of green to ultraviolet spectra of cell and tissue samples
6. US 20020058864 Reduction of spectral site to site variation
7. US 20020133080 Layered calibration standard for tissue sampling
8. US 20020010401 Pre- and post-processing of spectral data for calibration using multivariate analysis techniques
9. US 20010039483 Reduction of inter-subject variation via transfer standardization
Richard M. Levenson, James R. Mansfield, “Spectral imaging in medicine and biology” SPIE, San Jose, 2005, 2006
Chris van der Loos, James R. Mansfield “Multicolor immunohistochemistry: staining methods and multispectral imaging and analysis” National Society of Histotechnologists Annual Meeting, 2008, 2009, 2010, 2011, 2012